Journal: Cells

Article Title: Multiplexed Digital PCR Reference Gene Measurement for Genomic and Cell-Free DNA Analysis

doi: 10.3390/cells14191544

Figure Lengend Snippet: Reference gene measurement and HER2 ratio analysis of breast carcinoma cell line-derived hgDNA. ( A ) Reference gene panel was tested using HER2+ gDNA and its matched lymphoblast gDNA (HER2− gDNA). Error bar for the individual genes show range. The error bar for the average shows the expanded measurement uncertainty.; ( B ) HER2 multiplex panel was tested using HER2+ gDNA and its matched lymphoblast gDNA (HER2−). Symbols in black represent HER2+ gDNA, and symbols in grey represent HER2− gDNA. Circle, square, triangle, diamond, hexagon, and down-pointing triangle represent HBB, RPPH1, RPS27A, DCK, PMM1 and the average respectively. Each symbol shows individual measurements.

Article Snippet: Commercially available human genomic DNA (gDNA) isolated from the HCC1954 breast carcinoma cell line with HER2 copy number gain (ATCC CRL-2338D) and paired healthy control gDNA (ATCC CRL-2339D) isolated from the HCC1954 BL EBV-transformed lymphoblastoid cell line (both ATCC, Manassas, VA, USA) were also digested prior to dPCR application as described above.

Techniques: Derivative Assay, Multiplex Assay

Journal: Journal of the American Chemical Society

Article Title: Encapsulation of Gold-Based Anticancer Agents in Protease-Degradable Peptide Nanofilaments Enhances Their Potency

doi: 10.1021/jacs.2c09820

Figure Lengend Snippet: Cell Viability of gold anticancer agents, gold-loaded peptide nanofilaments ( PD+1 , PD+2 , AD+1 , AD+2 ), and peptides ( PD , AD ) in three human cell lines. Caki-1 (renal cancer), MDA-MB-231 (Triple Negative Breast Cancer), and IMR-90 (lung fibroblasts) incubated with drug-loaded peptide for 72 h. A) Compound 1 , B) Compound 2 . (* represents P <0.05, ** represents P <0.01, *** represents P <0.001).

Article Snippet: Human breast adenocarcinoma line MDA-MB-231 and human lung fibroblast line IMR-90 were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia, USA) and cultured with Dulbecco’s modified Eagle’s medium (DMEM; Fisher Scientific) containing 10 % fetal bovine serum, certified, heat inactivated, US origin (FBS; Fisher Scientific), 1 % minimum essential media (MEM) nonessential amino acids (NEAA; Fisher Scientific), and 1 % penicillin–streptomycin (PenStrep; Fisher Scientific).

Techniques: Incubation

Journal: bioRxiv

Article Title: Epigenetic Regulation of Inflammation by Dopamine in Primary Human Macrophages

doi: 10.64898/2026.01.21.700899

Figure Lengend Snippet: Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and DNA were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly increased methylated IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).

Article Snippet: Both high-methylated and low-methylated human genomic DNA (80-8061-HGHM5 and 80-8062-HGUM5 from EpigenDx) were bisulfite treated and used as controls for the primer sets.

Techniques: Derivative Assay, Control, Isolation, Gene Expression, DNA Methylation Assay, Expressing, Produced, Methylation